Journal:

Article Title: Progressor but not regressor skin tumours inhibit Langerhans' cell migration from epidermis to local lymph nodes

doi: 10.1046/j.1365-2567.1999.00751.x

Figure Lengend Snippet: LC division is similar in epidermis treated with control (CC) or tumour supernatant (TC) creams. Mice were treated by applying CC or TC topically for 5 consecutive days. They were simultaneously supplied with 5′ bromodeoxyuridine (BrdU) in their drinking water throughout the 5 days of treatment. Epidermal cell suspensions prepared from treated skin were immunohistochemically labelled for MHC class II and incorporated BrdU. The percentage of the LC which had incorporated BrdU into their nucleus was determined by counting total LC, and double positive LC. Mean±SEM shown for each group, N = 6 for each group. There was no significant difference in the percentage of divided LC within CC and TC samples (unpaired two-tailed Student's t-test).

Article Snippet: On day 6, the mice were sacrificed, the treated skin was removed, epidermal cell suspensions were prepared as described previously 10 and fixed with 0·2% paraformaldehyde in PBS for 5 min, then immunolabelled, as described by Holt et al ., 12 with rat anti-MHC class II antibody (TIB 120) and murine anti-BrdU (Becton Dickinson, San Jose, CA).

Techniques: Control, Two Tailed Test

Journal:

Article Title: Progressor but not regressor skin tumours inhibit Langerhans' cell migration from epidermis to local lymph nodes

doi: 10.1046/j.1365-2567.1999.00751.x

Figure Lengend Snippet: LC migration from the epidermis is inhibited by treatment with tumour supernatant. Mice were treated with control or tumour-supernatant-containing cream for 5 consecutive days. 24 hr later half of the treated mice (6 mice) were painted with either 0·1, 0·5% FITC or 3% TNCB, the other half received solvent alone. 18 hr later epidermal sheets were prepared and stained with anti-MHC class II antibodies to detect LC. LC numbers were determined using video image analysis. For each group, the percentage of LC which had migrated from the epidermis was calculated as described in Materials and Methods, mean±SEM shown for each group. To determine the statistical significance of FITC or TNCB -induced migration, each of these groups was compared to its respective group which received solvent alone (unpaired two-tailed Students t-test). *P < 0·01, **P < 0·001, NS not significant for FITC or TNCB compared to solvent-treated groups.

Article Snippet: On day 6, the mice were sacrificed, the treated skin was removed, epidermal cell suspensions were prepared as described previously 10 and fixed with 0·2% paraformaldehyde in PBS for 5 min, then immunolabelled, as described by Holt et al ., 12 with rat anti-MHC class II antibody (TIB 120) and murine anti-BrdU (Becton Dickinson, San Jose, CA).

Techniques: Migration, Control, Cream, Solvent, Staining, Two Tailed Test

Journal:

Article Title: Progressor but not regressor skin tumours inhibit Langerhans' cell migration from epidermis to local lymph nodes

doi: 10.1046/j.1365-2567.1999.00751.x

Figure Lengend Snippet: Tumour supernatant inhibits LC migration to local lymph nodes in response to 0·5% FITC. Groups of six mice were treated topically for 5 consecutive days with either control cream (CC) or tumour-supernatant-containing cream (TC). Twenty-four hours later 0·5% FITC was painted onto the treated skin. The number of LC that had migrated to draining auricular lymph nodes 18 hr following FITC was determined. Paraformaldehyde (0·1%)-fixed lymph node cells were labelled with anti-MHC class II–phycoerythrin (PE) and examined by flow cytometry for MHC class II and FITC double positivity. *denotes a statistically significant difference between control and tumour supernatant treated groups (P < 0·05, unpaired Student's t-test). Mean±SEM shown for each group.

Article Snippet: On day 6, the mice were sacrificed, the treated skin was removed, epidermal cell suspensions were prepared as described previously 10 and fixed with 0·2% paraformaldehyde in PBS for 5 min, then immunolabelled, as described by Holt et al ., 12 with rat anti-MHC class II antibody (TIB 120) and murine anti-BrdU (Becton Dickinson, San Jose, CA).

Techniques: Migration, Control, Cream, Flow Cytometry

Journal:

Article Title: Progressor but not regressor skin tumours inhibit Langerhans' cell migration from epidermis to local lymph nodes

doi: 10.1046/j.1365-2567.1999.00751.x

Figure Lengend Snippet: LC migration from the epidermis overlying the T7 progressor tumour is inhibited. Groups of 16 HRA:Skh-1 mice were transplanted with 2×105 T7 tumour cells into each side of the dorsal flank, and the tumours were allowed to grow to a diameter of 10 mm. Control groups did not receive tumour cells. Half of the mice in each group (eight mice) were painted with either 1% FITC, 1% FITC and 0·5% TNCB, or 1% FITC and 3% TNCB. The other half of each group received solvent alone. Eighteen hours later epidermal sheets were prepared and stained for LC with anti-MHC class II antibodies. LC numbers were determined using video image analysis. For each group, the percentage of LC which had migrated from the epidermis was calculated as described in Materials and Methods, mean±SEM shown for each group. To determine the statistical significance of FITC- or FITC+TNCB-induced migration, each of these groups was compared to its respective group which received solvent alone (unpaired two-tailed Students t-test). *P < 0·01, **P < 0·001, NS not significant for FITC or FITC+TNCB compared to solvent-treated groups.

Article Snippet: On day 6, the mice were sacrificed, the treated skin was removed, epidermal cell suspensions were prepared as described previously 10 and fixed with 0·2% paraformaldehyde in PBS for 5 min, then immunolabelled, as described by Holt et al ., 12 with rat anti-MHC class II antibody (TIB 120) and murine anti-BrdU (Becton Dickinson, San Jose, CA).

Techniques: Migration, Control, Solvent, Staining, Two Tailed Test

Journal:

Article Title: Progressor but not regressor skin tumours inhibit Langerhans' cell migration from epidermis to local lymph nodes

doi: 10.1046/j.1365-2567.1999.00751.x

Figure Lengend Snippet: LC migration is inhibited from the epidermis overlying progressor but not regressor tumours. Groups of 16 BALB/c nu/nu mice were transplanted with progressor or regressor tumour cell lines, and the tumours were allowed to grow to a diameter of 10 mm. Other groups did not receive tumour cells and served as non-tumour-bearing controls. Half of the mice were painted with 1% FITC, the other half received solvent alone. Eighteen hours later epidermal sheets were prepared and stained for LC with anti-MHC class II antibodies. LC numbers were determined using video image analysis. For each group, the percentage of LC which had migrated from the epidermis was calculated as described in Materials and Methods, mean±SEM are shown for each group. To determine the statistical significance of FITC-induced migration, each of these groups was compared to its respective group which received solvent alone (unpaired two-tailed Students t-test). *P < 0·05, **P < 0·01, NS not significant for FITC-compared to solvent-treated groups.

Article Snippet: On day 6, the mice were sacrificed, the treated skin was removed, epidermal cell suspensions were prepared as described previously 10 and fixed with 0·2% paraformaldehyde in PBS for 5 min, then immunolabelled, as described by Holt et al ., 12 with rat anti-MHC class II antibody (TIB 120) and murine anti-BrdU (Becton Dickinson, San Jose, CA).

Techniques: Migration, Solvent, Staining, Two Tailed Test